%0 Journal Article
%T 煤矿土壤甲烷氧化菌的筛选及其培养条件优化的初步研究
%A 侯彤
%A 周生芳
%A 朱红威
%A 许宁
%A 陶秀祥
%J 洁净煤技术
%D 2008
%X 从江苏徐州夹河煤矿土壤中分离筛选到一株好氧型甲烷氧化细菌,命名为ZMH。此菌摇床培养10d后甲烷浓度由35·94%下降到10·00%,甲烷的利用率达64·18%。除甲烷以外,该菌还能利用葡萄糖、蔗糖、麦芽糖作为碳源进行生长;另外,此菌可利用多种化合物作为氮源,如NO3--N、NH4+-N和酵母膏,还可以利用空气中的氮气。对该菌的培养条件研究发现其生长的pH范围为5·5~9·0,最适pH值为6·5;适宜的生长温度为30~34℃。 A strain of aerobic methane-oxidizing bacteria was isolated from JiaHe coal(Xuzhou),and was designated as Strain ZMH. After 10 days incubation of ZMH,CH4 concentration decreased from 35.94% to 10.00%,and the utilization rate of CH4 reached 64.18%. The results indicated that this isolate could not only utilize methane as carbon sources,but also utilized other growth substrates such as glucose,sucrose and maltose. Nitrogen sources were tested,demonstrating that ZMH could use NO-3-N、NH+4-N and yeast extract paste,it could also use nitrogen in the air. The optimal pH for growth was 5.5～9.0,with the fastest growth occurring at pH 6.5. Growth occurred at temperatures between 30 and 34℃
%K 甲烷氧化菌
%K 分离纯化
%K 甲烷
%K 培养条件 methane-oxidizing bacteria
%K isolation and purification
%K methane
%K culture conditions
%U http://www.jjmjs.com.cn/WKD/WebPublication/paperDigest.aspx?paperID=3d574ccf-4d14-433b-8388-2595a60ff045