%0 Journal Article
%T Current Strategies and Challenges for Purification of Cardiomyocytes Derived from Human Pluripotent Stem Cells
%A Kiwon Ban
%A Seongho Bae
%A Young-sup Yoon
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.19427
%X Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) are considered a most promising option for cell-based cardiac repair. Hence, various protocols have been developed for differentiating hPSCs into CMs. Despite remarkable improvement in the generation of hPSC-CMs, without purification, these protocols can only generate mixed cell populations including undifferentiated hPSCs or non-CMs, which may elicit adverse outcomes. Therefore, one of the major challenges for clinical use of hPSC-CMs is the development of efficient isolation techniques that allow enrichment of hPSC-CMs. In this review, we will discuss diverse strategies that have been developed to enrich hPSC-CMs. We will describe major characteristics of individual hPSC-CM purification methods including their scientific principles, advantages, limitations, and needed improvements. Development of a comprehensive system which can enrich hPSC-CMs will be ultimately useful for cell therapy for diseased hearts, human cardiac disease modeling, cardiac toxicity screening, and cardiac tissue engineering.
%K Cardiomyocytes
%K hPSCs
%U http://www.thno.org/v07p2067.htm