%0 Journal Article
%T MicroRNA-449a Inhibits Tumor Metastasis through AKT/ERK1/2 Inactivation by Targeting Steroid Receptor Coactivator (SRC) in Endometrial Cancer
%A An-Yue Wu
%A Cong Xu
%A Ke-Qi Song
%A Li-Hua Qiu
%A Wen Di
%A Wen-Jing Wang
%A Xia Yin
%A Yuan Hu
%A Zu-Bei Hong
%J Journal of Cancer
%D 2019
%I Ivyspring International Publisher
%R 10.7150/jca.27748
%X Endometrial cancer represents the leading frequency in gynecological malignancy in developed countries. Even with early detection, metastasis and recurrence remain the main reasons for a high death rate. MicroRNA-449a (miR-449a) has been reported to function as a tumor suppressor, yet the role of miR-449a in endometrial cancer metastasis has not been investigated. The present study identified that miR-449a was downregulated in advanced endometrial cancer. Overexpression of miR-449a decreased the migration and invasion of KLE and AN3CA endometrial cancer cells. Using luciferase reporter assays, we identified that miR-449a directly targeted the steroid receptor coactivator (SRC) by binding to sites in the 3£¿ untranslated regions. Elevated expressions of SRC have been witnessed in advanced endometrial cancer tissues and have promoted tumor metastasis. We also identified that the suppressive effect of miR-449a on metastasis could be mediated by downregulating SRC and that miR-449a could suppress AKT and ERK1/2 pathway activation in endometrial cancer cells. These findings contribute to the current understanding of the function of miR-449a in endometrial cancer.
%K Endometrial cancer
%K MicroRNA-449a
%K SRC
%K Metastasis
%U http://www.jcancer.org/v10p0547.htm