%0 Journal Article
%T MiR27a Promotes the Development of Macrophage-like Characteristics in 3T3-L1 Preadipocytes
%A Grant M. Hatch
%A Jing Zhang
%A Li Chen
%A Meishuang Zhang
%A Ming Zhang
%A Shidong Sheng
%A Wenyou Zhang
%A Zhanqiang Zhang
%J International Journal of Biological Sciences
%D 2018
%R 10.7150/ijbs.26274
%X Recruitment and polarization of classically activated (M1) macrophages within adipose tissue contribute to chronic low-grade inflammation in obesity. Adipose tissue precursor cells exhibit the capacity to develop macrophage-like characteristics and adipocyte-derived miR27a is known to promote reprogramming of somatic cells. It was unknown whether exogenous addition of miR27a promote the development of macrophage-like characteristics of adipose precursor cells. We examined macrophage surface antigen, phagocytosis and migration ability in 3T3-L1 preadipocytes transfected with miR27a mimics. Transfection of 3T3-L1 preadipocytes with miR27a mimics increased phagocytosis and migration and increased the number of cells expressing the macrophage makers F4/80 and MHC compared to controls. M2 and CD206 macrophage markers were unaltered. In addition, transfection of 3T3-L1 preadipocytes with miR27a mimics reduced PPAR¦Ã expression, activated NF-¦ÊB and promoted secretion of the inflammatory cytokines MCP-1, TNF-¦Á and IL-1¦Â compared to controls. The level of anti-inflammatory factors Arg-1, IL-10, Ym1 and Fizz1 were unaltered. Secretion of miR27a was increased in conditioned medium prepared from palmitic acid-treated differentiated 3T3-L1 adipocytes compared to controls. Incubation of 3T3-L1 preadipocytes with this conditioned medium increased phagocytosis and migration compared to controls. Finally, conditioned medium prepared from differentiated 3T3-L1 adipocytes transfection with miR27a inhibitors reduced phagocytosis and migration in 3T3-L1 preadipocytes compared to controls. The data indicate that PPAR¦Ã agonists may reverse the activation of NF-¦ÊB pathway mediated by miR27a overexpression and reduce phagocytosis and migration of adipose precursor cells. In addition, miR27a may promote the development of macrophage-like characteristics in 3T3-L1 preadipocytes.
%K obesity
%K miR27a
%K inflammation
%K preadipocytes
%K 3T3 cells
%K macrophage-like
%U http://www.ijbs.com/v14p1599.htm