%0 Journal Article
%T 西藏自治区色季拉山高寒草甸牧草根腐线虫的鉴定<br>Identification of lesion nematode in alpine cold meadow of Color Gerahs Mountain,Tibet
%A 王宏洪
%A 卓侃
%A 薛会英
%J 华中农业大学学报
%D 2019
%X 在西藏自治区色季拉山高寒草甸土壤线虫群落调查期间，从高山嵩草（Kobresia pygmaea）根际土壤中分离得到一种根腐线虫。通过形态特征观察和分子序列比对，将其鉴定为刻痕短体线虫（Pratylenchus crenatus）。其主要特征：唇环3个，口针长15.6~16.2 μm，口针基部球前缘略凹，侧线6条，V值为82.5~83.2，尾圆柱形，末端环纹清晰。对该线虫rDNA的3个区段（18S部分序列、28S D2D3区和ITS区）进行特异性扩增和测序，分别得到902、774和1 013 bp的序列。将所获得的序列在GenBank数据库上进行BLAST比对，结果显示：此线虫群体与刻痕短体线虫群体的相似度分别为97.4%~99.8%、97.4%~97.8%和90.2%~99.8%。贝叶斯推断法构建的系统进化树显示：本研究的线虫群体与刻痕短体线虫聚为高度支持的分支，进一步确定此线虫群体为刻痕短体线虫。本研究获得刻痕短体线虫中国群体的分子数据。该刻痕短体线虫是西藏自治区的新纪录种。高山嵩草是刻痕短体线虫的新寄主。<br>Degenerated primers were designed according to the conserved regions of NBS LRR domain from known plant resistance genes. Then these primers were used for amplification of the NBS LRR analogs from Lycopersicon esculentum. Sequence analysis showed that 5 fragments contained the resistance gene analogs,two of which have the ORF region and conserved NBS LRR domain. F5 8 (MG860907) shared 94% identity with the resistance genes of Mi 1, and F5 20 (MG860908) shared 96% identity with the resistance genes of Hero. The quantitative real time PCR results showed that expression of F5 8 and F5 20 was changed after Meloidogyne incognita infection,suggesting that F5 8 and F5 20 might be related with nematode infection.
%K 刻痕短体线虫 高山嵩草 形态特征 分子序列分析 新纪录<br>Lycopersicon esculentum  Meloidogyne incognita resistance gene analogs (RGAs) NBS LRR
%U http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20190109&flag=1