%0 Journal Article
%T Stable contacts of na£żve CD4 T cells with migratory dendritic cells are ICAM-1-dependent but dispensable for proliferation in vivo
%A Ofir Atrakchi
%A Ronen Alon
%A Sara W Feigelson
%A Stav Kozlovski
%A Ziv Shulman
%J Cell Adhesion & Migration
%D 2019
%R https://doi.org/10.1080/19336918.2019.1644857
%X ABSTRACT It is unclear if na£żve T cells require dendritic cell ICAMs to proliferate inside lymph nodes. To check if and when CD4 lymphocytes use ICAMs on migratory DCs, wild-type and ICAM-1 and 2 double knock out bone marrow-derived DCs pulsed with saturating levels of an OT-II transgene-specific ovalbumin-derived peptide were co-transferred into skin-draining lymph nodes. Intravital imaging of OT-II lymphocytes entering these lymph nodes revealed that ICAM-1 and £ż2 deficient migratory DCs formed fewer stable conjugates with OT-II lymphocytes but promoted normal T cell proliferation. DC ICAMs were also not required for unstable TCR-dependent lymphocyte arrests on antigen presenting migratory DCs. Thus, rare antigen-stimulated ICAM-stabilized T-DC conjugates are dispensable for CD4 lymphocyte proliferation inside lymph nodes
%U https://www.tandfonline.com/doi/full/10.1080/19336918.2019.1644857