%0 Journal Article
%T Identification and characterization of cryoprotective peptides extracted from silver carp (Hypophthalmichthys molitrix) hydrolysates
%A Faxiang Wang
%A Jian Yu
%A Qingjing Liu
%A Xianghong Li
%A Yongle Liu
%J International Journal of Food Properties
%D 2019
%R https://doi.org/10.1080/10942912.2019.1622563
%X ABSTRACT The cryoprotective effects on surimi matrix of hydrolysates with different hydrolyzing times prepared from silver carp using Protamex were compared with commercial sorbitol-sucrose cryoprotectants. Cryoprotective assays showed that FPH-30 (hydrolysate with 30 min hydrolyzing) was a more effective cryoprotectant than other hydrolysates, because the FPH-30 group displayed lower salt-soluble protein extractability loss (29.90 ¡À 0.84%), less actomyosin Ca2+-ATPase activity decrease (48.85 ¡À 2.56%) and unfrozen water content decrease (10.39 ¡À 0.63%) after six freeze-thaw cycles. To exploit industrial utilizations, FPH-30 was further purified with ultrafiltration membranes. The cryoprotective activity of the fraction (<3 kDa) was superior to the others by reducing the salt-soluble protein extractability loss (27.21 ¡À 0.98%) and impeding the actomyosin Ca2+-ATPase activity decrease (30.54 ¡À 1.21%). Mass spectrometry analysis showed that the fraction (<3 kDa) was mainly composed of three peptides of the sequences GVDNPGHPFIM(T) and IITNWDDMEK. The results clearly support that silver carp could be a potential source of cryoprotectant peptides for industrial applications
%U https://www.tandfonline.com/doi/full/10.1080/10942912.2019.1622563