%0 Journal Article
%T Effect of electrical stimulation combined with graphene-oxide-based membranes on neural stem cell proliferation and differentiation
%A Chuan Fu
%A Su Pan
%A Weijian Kong
%A Xiaoyu Yang
%A Yue Ma
%A Zhiping Qi
%J Artificial Cells, Nanomedicine, and Biotechnology
%D 2019
%R https://doi.org/10.1080/21691401.2019.1613422
%X Abstract The combination of composite nerve materials prepared using degradable polymer materials with biological or physical factors has received extensive attention as a means to treat nerve injuries. This study focused on the potential application of graphene oxide (GO) composite conductive materials combined with electrical stimulation (ES) in nerve repair. A conductive poly(L-lactic-co-glycolic acid) (PLGA)/GO composite membrane was prepared, and its properties were tested using a scanning electron microscope (SEM), a contact angle meter, and a mechanical tester. Next, neural stem cells (NSCs) were planted on the PLGA/GO conductive composite membrane and ES was applied. NSC proliferation and differentiation and neurite elongation were observed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, immunofluorescence, and PCR, respectively. The results showed that the PLGA/GO membrane had good hydrophilicity, mechanical strength, and protein adsorption. ES combined with the PLGA/GO membrane significantly promoted NSC proliferation and neuronal differentiation on the material surface and promoted significant neurite elongation. Our results suggest that ES combined with GO-related conductive composite materials can be used as a new therapeutic combination to treat nerve injuries
%U https://www.tandfonline.com/doi/full/10.1080/21691401.2019.1613422