%0 Journal Article
%T Transforming Growth Factor Beta 3-Loaded Decellularized Equine Tendon Matrix for Orthopedic Tissue Engineering
%J International Journal of Molecular Sciences | An Open Access Journal from MDPI
%D 2019
%R https://doi.org/10.3390/ijms20215474
%X Transforming growth factor beta 3 (TGF¦Â3) promotes tenogenic differentiation and may enhance tendon regeneration in vivo. This study aimed to apply TGF¦Â3 absorbed in decellularized equine superficial digital flexor tendon scaffolds, and to investigate the bioactivity of scaffold-associated TGF¦Â3 in an in vitro model. TGF¦Â3 could effectively be loaded onto tendon scaffolds so that at least 88% of the applied TGF¦Â3 were not detected in the rinsing fluid of the TGF¦Â3-loaded scaffolds. Equine adipose tissue-derived multipotent mesenchymal stromal cells (MSC) were then seeded on scaffolds loaded with 300 ng TGF¦Â3 to assess its bioactivity. Both scaffold-associated TGF¦Â3 and TGF¦Â3 dissolved in the cell culture medium, the latter serving as control group, promoted elongation of cell shapes and scaffold contraction ( p < 0.05). Furthermore, scaffold-associated and dissolved TGF¦Â3 affected MSC musculoskeletal gene expression in a similar manner, with an upregulation of tenascin c and downregulation of other matrix molecules, most markedly decorin ( p < 0.05). These results demonstrate that the bioactivity of scaffold-associated TGF¦Â3 is preserved, thus TGF¦Â3 application via absorption in decellularized tendon scaffolds is a feasible approach. View Full-Tex
%U https://www.mdpi.com/1422-0067/20/21/5474