%0 Journal Article
%T Evaluation of a Urine-Based Rapid Molecular Diagnostic Test with Potential to Be Used at Point-of-Care for Pulmonary Tuberculosis: Cape Town Cohort
%A Christof Geldmacher
%A Eric Rivera-Milla
%A Ines Labugger
%A Keertan Dheda
%A Krutarth Patel
%A Maria Wesolowski
%A Matilde Nagel
%A Michael Hoelscher
%A Stefan Dees
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2017.11.005
%X Tuberculosis (TB) diagnosis among sputum-scarce patients is time consuming. Thus,
a nonsputum diagnostic alternative is urgently needed. The Mycobacterium tuberculosis¨Cspecific
transrenal (Tr) DNA from urine is a potential target for TB diagnostics. In this study,
a new urine-based Tr-DNA molecular assay was evaluated for diagnosis of pulmonary
tuberculosis among 428 adults suspected of having pulmonary TB (164 HIV positive,
263 HIV negative) from Cape Town, South Africa. Tr-DNA was isolated from 4 mL of EDTA
urine, and a rapid, double-stranded, primer-based PCR method was performed targeting
the Mycobacterium tuberculosis¨Cspecific direct repeat region.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30247-7/fulltext