%0 Journal Article
%T Evaluation of Diagnostic Utility of a High-Risk Human Papillomavirus PCR Test on Formalin-Fixed, Paraffin-Embedded Head and Neck Tumor Tissues
%A Albert N. Huho
%A Nour Yadak
%A Shangxin Yang
%A Th¨¨r¨¦se J. Bocklage
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2017.11.008
%X The increasing prevalence of high-risk human papillomavirus (HR-HPV)¨Cassociated head
and neck squamous cell carcinoma (HNSCC) has prompted strong clinical demands for
detecting HR-HPV directly in the tumor. Although p16 immunohistochemistry (IHC) has
been the standard testing method, it has limitations including false positivity, lack
of sensitivity in low tumor cell samples such as fine-needle aspirate (FNA), and its
subjectivity. We developed a modified method based on a commercial automated HR-HPV
PCR assay and evaluated the performance characteristics and the diagnostic utility
of this assay for direct HR-HPV detection in the HNSCC samples.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30437-3/fulltext