%0 Journal Article
%T Transferring a Quantitative Molecular Diagnostic Test to Multiple Real-Time Quantitative PCR Platforms
%A Claudia G¨¹rtler
%A Heike Steinh£¿user
%A Kornelia Schlombs
%A Mark Laible
%A Shujin Liu
%A Ugur Sahin
%A Wolfgang Schwabe
%A Xingmin Li
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.02.004
%X Quantitative gene expression assays are increasingly used for diagnosis and research,
but are often restricted to specific instrumentation. We propose a robust technical
and statistical framework that enables transferring of established real-time quantitative
PCR assays across real-time quantitative PCR platforms without compromising analytical
and clinical validity. The feasibility of our approach was tested on MammaTyper, an
in£¿vitro diagnostic assay that quantifies breast cancer biomarkers and dichotomizes
results according to cutoff points.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30524-X/fulltext