%0 Journal Article
%T Molecular Analysis of Gene Fusions in Bone and Soft Tissue Tumors by Anchored Multiplex PCR¨CBased Targeted Next-Generation Sequencing
%A Anne-Marie Cleton-Jansen
%A Arjen H.G. Cleven
%A Dina Ruano
%A Judith V.M.G. Bov¨¦e
%A Karoly Szuhai
%A Suk Wai Lam
%A Tom van Wezel
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.05.007
%X Molecular assays for translocation detection in bone and soft tissue tumors have gradually
been incorporated into routine diagnostics. However, conventional methods such as
fluorescence in situ hybridization (FISH) and reverse transcriptase-PCR come with
several drawbacks. In this study, the applicability of a novel technique termed anchored
multiplex PCR (AMP) for next-generation sequencing (NGS), using the Archer FusionPlex
Sarcoma kit, aimed at 26 genes, was evaluated and compared with FISH and reverse transcriptase-PCR.
%U https://jmd.amjpathol.org/article/S1525-1578(18)30058-8/fulltext