%0 Journal Article
%T Pre-Analytical Handling Conditions and Small RNA Recovery from Urine for miRNA Profiling
%A Alix Ashare
%A Bruce A. Stanton
%A Carol S. Ringelberg
%A David A. Armstrong
%A Haley F. Hazlett
%A John A. Dessaint
%A Louisa Howard
%A Mark A. Cervinski
%A Moemen A.K. Abdalla
%A Roxanna L. Barnaby
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.04.003
%X There are currently no standardized protocols for pre-analytical handling of urine
to best preserve small RNA for miRNA profiling studies. miRNA is an attractive candidate
as a potential biomarker because of the high level of stability in body fluids and
its ability to be quantified on multiple high-throughput platforms. We present a comparison
of small RNA recovery and stability in urine under alternate pre-analytical handling
conditions and extend recommendations on what conditions optimize yield of miRNA from
cell-free urine and urine extracellular vesicles (EVs).
%U https://jmd.amjpathol.org/article/S1525-1578(18)30005-9/fulltext