%0 Journal Article
%T Clinical Validation and Implementation of a Targeted Next-Generation Sequencing Assay to Detect Somatic Variants in Non-Small Cell Lung, Melanoma, and Gastrointestinal Malignancies
%A Annie S. Morrison
%A Anthony A. Gal
%A Bassel F. El-Rayes
%A Charles E. Hill
%A David H. Lawson
%A Debra F. Saxe
%A Fadlo R. Khuri
%A Gabriel L. Sica
%A Geoffrey H. Smith
%A H. Clifford Sullivan
%A Jason Wang
%A Karen P. Mann
%A Keith A. Delman
%A Kevin E. Fisher
%A Linsheng Zhang
%A Malania M. Wilson
%A Michael R. Rossi
%A N. Volkan Adsay
%A Ragini R. Kudchadkar
%A Rathi N. Pillai
%A Scott Newman
%A Serdar Balci
%A Suresh S. Ramalingam
%A Taofeek K. Owonikoko
%A Thomas M. Schneider
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2015.11.006
%X We tested and clinically validated a targeted next-generation sequencing (NGS) mutation
panel using 80 formalin-fixed, paraffin-embedded (FFPE) tumor samples. Forty non-small
cell lung carcinoma (NSCLC), 30 melanoma, and 30 gastrointestinal (12 colonic, 10
gastric, and 8 pancreatic adenocarcinoma) FFPE samples were selected from laboratory
archives. After appropriate specimen and nucleic acid quality control, 80 NGS libraries
were prepared using the Illumina TruSight tumor (TST) kit and sequenced on the Illumina
MiSeq.
%U https://jmd.amjpathol.org/article/S1525-1578(15)00276-7/fulltext