%0 Journal Article
%T Validation of a Customized Bioinformatics Pipeline for a Clinical Next-Generation Sequencing Test Targeting Solid Tumor¨CAssociated Variants
%A Charles E. Hill
%A Geoffrey H. Smith
%A Linsheng Zhang
%A Michael R. Rossi
%A Thomas Schneider
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.01.007
%X Bioinformatic analysis is an integral and critical part of clinical next-generation
sequencing. It is especially challenging for some pipelines to consistently identify
insertions and deletions. We present the validation of an open source tumor amplicon
pipeline (OTA-pipeline) for clinical next-generation sequencing targeting solid tumor¨Cassociated
variants. Raw data generated from 557 TruSight Tumor 26 samples and in silico data
were analyzed by the OTA-pipeline and legacy pipeline and compared.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30335-5/fulltext