%0 Journal Article
%T Accurate Sample Assignment in a Multiplexed, Ultrasensitive, High-Throughput Sequencing Assay for Minimal Residual Disease
%A Edward Mountjoy
%A Gary Wright
%A Helen Williamson
%A Jack Bartram
%A Jeremy Hancock
%A John Moppett
%A Mike Hubank
%A Nick Goulden
%A Tony Brooks
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2016.02.008
%X High-throughput sequencing (HTS) (next-generation sequencing) of the rearranged Ig
and T-cell receptor genes promises to be less expensive and more sensitive than current
methods of monitoring minimal residual disease (MRD) in patients with acute lymphoblastic
leukemia. However, the adoption of new approaches by clinical laboratories requires
careful evaluation of all potential sources of error and the development of strategies
to ensure the highest accuracy. Timely and efficient clinical use of HTS platforms
will depend on combining multiple samples (multiplexing) in each sequencing run.
%U https://jmd.amjpathol.org/article/S1525-1578(16)30045-9/fulltext