%0 Journal Article
%T A Novel and Reliable Method to Detect Microsatellite Instability in Colorectal Cancer by Next-Generation Sequencing
%A Chenglin Liu
%A Chenhan Zhong
%A Dong Xu
%A Jinghong Xu
%A Lizhen Zhu
%A Wanglong Deng
%A Xuefeng Fang
%A Yanqin Huang
%A Ying Yuan
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2017.11.007
%X Two types of molecular tests have been established to assess the deficiency of the
DNA mismatch repair (MMR) system: microsatellite instability (MSI) and immunohistochemical
(IHC) analysis. We have developed a reliable method to analyze the MSI status by next-generation
sequencing (NGS) based on read-count distribution. A total of 91 patients with primary
colorectal cancer were recruited. These patients included 54 cases with loss of expression
of any MMR protein in IHC, suggesting deficient MMR (dMMR), and 37 cases of colorectal
cancer with staining of all four MMR proteins in IHC, suggesting proficient MMR in
the sample after surgery.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30369-0/fulltext