%0 Journal Article
%T Evaluation of a Pan-Leishmania Spliced-Leader RNA Detection Method in Human Blood and Experimentally Infected Syrian Golden Hamsters
%A Dimitri Bult¨¦
%A Dorien Mabille
%A Eline Eberhardt
%A Fabiana Alves
%A Guy Caljon
%A Jane Mbui
%A Lieselotte Van Bockstal
%A Louis Maes
%A Magali Van den Kerkhof
%A Paul Cos
%A Peter Delputte
%A Sarah Hendrickx
%A S¨¦verine Monnerat
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2017.12.003
%X Several methods have been developed for the detection of Leishmania, mostly targeting
the minicircle kinetoplast DNA (kDNA). A new RNA real-time quantitative PCR (qPCR)
assay was developed targeting the conserved and highly expressed spliced-leader (SL)
mini-exon sequence. This study compared the limits of detection of various real-time
PCR assays in hamsters infected with Leishmania infantum, in spiked human blood, and
in clinical blood samples from visceral leishmaniasis patients. The SL-RNA assay showed
an excellent analytical sensitivity in tissues (0.005 and 0.002 parasites per mg liver
and spleen, respectively) and was not prone to false-positive reactions.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30151-4/fulltext