%0 Journal Article
%T Optimal Reference Gene Selection for Expression Studies in Human Reticulocytes
%A Anu Aggarwal
%A Deepak Bansal
%A Ganesh K. Viswanathan
%A ManUpdesh S. Sachdeva
%A Manu Jamwal
%A Pankaj Malhotra
%A Prashant Sharma
%A Reena Das
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.01.009
%X Reference genes are indispensable for normalizing mRNA levels across samples in real-time
quantitative PCR. Their expression levels vary under different experimental conditions
and because of several inherent characteristics. Appropriate reference gene selection
is thus critical for gene-expression studies. This study aimed at selecting optimal
reference genes for gene-expression analysis of reticulocytes and at validating them
in hereditary spherocytosis (HS) and ¦Â-thalassemia intermedia (¦ÂTI) patients.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30431-2/fulltext