%0 Journal Article
%T Development and Verification of an RNA Sequencing (RNA-Seq) Assay for the Detection of Gene Fusions in Tumors
%A Amber M. McDonald
%A Asha A. Nair
%A Benjamin R. Kipp
%A Brittany C. Thomas
%A Eric W. Klee
%A Gavin R. Oliver
%A Jadee L. Neff
%A Jaime I. Davila
%A Jennifer L. Winters
%A Jesse S. Voss
%A Jessica R. Balcom
%A Jin Jen
%A Kandelaria M. Rumilla
%A Kevin C. Halling
%A Long Jin
%A Numrah Fadra
%A Rebecca N. Wehrs
%A Robert B. Jenkins
%A Rondell P. Graham
%A Umut Aypar
%A Xianglin Wu
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.03.007
%X We assessed the performance characteristics of an RNA sequencing (RNA-Seq) assay designed
to detect gene fusions in 571 genes to help manage patients with cancer. Polyadenylated
RNA was converted to cDNA, which was then used to prepare next-generation sequencing
libraries that were sequenced on an Illumina HiSeq 2500 instrument and analyzed with
an in-house developed bioinformatic pipeline. The assay identified 38 of 41 gene fusions
detected by another method, such as fluorescence in situ hybridization or RT-PCR,
for a sensitivity of 93%.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30541-X/fulltext