%0 Journal Article
%T Development of a Fluorescence in Situ Hybridization Probe for Detecting IKZF1 Deletion Mutations in Patients with Acute Lymphoblastic Leukemia
%A Chikara Shimizu
%A Daigo Hashimoto
%A Junichi Hashiguchi
%A Kaoru Kahata
%A Kohei Okada
%A Masahiro Onozawa
%A Masahisa Tsuji
%A Masao Nakagawa
%A Satoshi Oguri
%A Shinichi Fujisawa
%A Takanori Teshima
%A Takeshi Kondo
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.02.005
%X Intragenic deletion of IKZF1 is a recurrent genomic alteration in acute lymphoblastic
leukemia. The deletions are mediated by illegitimate variable(diversity)joining recombination
via cryptic recombination signal sequences (RSSs). We developed a fluorescence in
situ hybridization (FISH) probe set that can detect any type of IKZF1 deletion, including
the commonly deleted exon 4 to 7 region. The probe set consists of a designed probe
for the commonly deleted region (Cy3; red) and a bacterial artificial chromosomes
clone probe for detecting the 3กไ flanking region (Spectrum Green).
%U https://jmd.amjpathol.org/article/S1525-1578(17)30412-9/fulltext