%0 Journal Article
%T Development and Validation of a Template-Independent Next-Generation Sequencing Assay for Detecting Low-Level Resistance-Associated Variants of Hepatitis C Virus
%A Bo Wei
%A Diane Levitan
%A Fred Lahser
%A John Kang
%A Lei Chen
%A Matthew Marton
%A Miho Kibukawa
%A Ping Qiu
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2016.04.001
%X To develop hepatitis C virus (HCV) direct-acting antiviral (DAA) drugs that can treat
most HCV genotypes and offer higher barriers for treatment-resistant mutations, it
is important to study resistance-associated variants (RAVs). Current commercially
available RAV detection assays rely on genotype- or subtype-specific template-dependent
PCR amplification. These assays are limited to genotypes and subtypes that are often
prevalent in developed countries because of availability of public sequence databases.
%U https://jmd.amjpathol.org/article/S1525-1578(16)30063-0/fulltext