%0 Journal Article
%T A Multiplexed Amplicon Approach for Detecting Gene Fusions by Next-Generation Sequencing
%A Abigail I. Wald
%A Andrea Warrick
%A Carol Beadling
%A Christopher L. Corless
%A Marina N. Nikiforova
%A Shan Zhong
%A Tanaya L. Neff
%A Yuri E. Nikiforov
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2015.10.002
%X Chromosomal rearrangements that result in oncogenic gene fusions are clinically important
drivers of many cancer types. Rapid and sensitive methods are therefore needed to
detect a broad range of gene fusions in clinical specimens that are often of limited
quantity and quality. We describe a next-generation sequencing approach that uses
a multiplex PCR-based amplicon panel to interrogate fusion transcripts that involve
19 driver genes and 94 partners implicated in solid tumors. The panel also includes
control assays that evaluate the 3กไ/5กไ expression ratios of 12 oncogenic kinases,
which might be used to infer gene fusion events when the partner is unknown or not
included on the panel.
%U https://jmd.amjpathol.org/article/S1525-1578(15)00257-3/fulltext