%0 Journal Article
%T The Clinical Implications of Inconsistently Methylated Results from Glioblastoma MGMT Testing by Replicate Methylation-Specific PCR
%A Daniel Xia
%A David A. Reardon
%A Jacqueline L. Bruce
%A Neal I. Lindeman
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2016.06.009
%X The methylation status of the promoter of the O6-methylguanine DNA methyltransferase
gene (MGMT) is an established prognostic and predictive biomarker of glioblastoma
(GBM). At the Center for Advanced Molecular Diagnostics, MGMT testing is performed
by methylation-specific PCR with multiple replicates, leading to three types of reportable
results: methylated, unmethylated, and inconsistently methylated. An inconsistently
methylated result is reported when a methylated peak is seen in some but not all of
the PCR replicates from a single DNA sample.
%U https://jmd.amjpathol.org/article/S1525-1578(16)30139-8/fulltext