%0 Journal Article
%T Assessment of Capture and Amplicon-Based Approaches for the Development of a Targeted Next-Generation Sequencing Pipeline to Personalize Lymphoma Management
%A Andrew J. Mungall
%A Barbara Meissner
%A Christian Steidl
%A Daisuke Ennishi
%A David W. Scott
%A Elizabeth A. Chavez
%A Fong Chun Chan
%A Hennady P. Shulha
%A Joseph M. Connors
%A Marco A. Marra
%A Martin R. Jones
%A Merrill Boyle
%A Randy D. Gascoyne
%A Robert Kridel
%A Stacy S. Hung
%A Susana Ben-Neriah
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2017.11.010
%X Targeted next-generation sequencing panels are increasingly used to assess the value
of gene mutations for clinical diagnostic purposes. For assay development, amplicon-based
methods have been preferentially used on the basis of short preparation time and small
DNA input amounts. However, capture sequencing has emerged as an alternative approach
because of high testing accuracy. We compared capture hybridization and amplicon sequencing
approaches using fresh-frozen and formalin-fixed, paraffin-embedded tumor samples
from eight lymphoma patients.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30425-7/fulltext