%0 Journal Article
%T Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Endonuclease Cas9¨CMediated Homology-Independent Integration for Generating Quality Control Materials for Clinical Molecular Genetic Testing
%A Guigao Lin
%A Jiehong Xie
%A Jinming Li
%A Kuo Zhang
%A Rongxue Peng
%A Yanxi Han
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.02.002
%X Genome-edited human cell lines are important resources for producing quality control
materials for clinical molecular genetic testing. Generating cell lines with defined
mutations through homology-directed repair¨Cbased methods are inefficient and can lead
to unwanted insertions and deletions in the target loci. Nonhomologous end joining
in the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
endonuclease Cas9 (Cas9) system was harnessed to generate genome-engineered cell lines
harboring target mutations.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30489-0/fulltext