%0 Journal Article %T Detection of Circulating BRAFV600E in Patients with Papillary Thyroid Carcinoma %A Aislyn P. Schalck %A Carrie C. Lubitz %A David J. Panka %A Hyunsuk Suh %A Konstantinos P. Economopoulos %A Lori J. Wirth %A M. Jordana Bernasconi %A Peter M. Sadow %A Ryan J. Sullivan %A Samuel E. Donovan %A Sareh Parangi %A Tammy M. Holm %A Viswanath Gunda %J The Journal of Molecular Diagnostics %D 2016 %R 10.1016/j.jmoldx.2015.08.003 %X BRAFV600E is a common mutation in papillary thyroid carcinoma (PTC) correlated with aggressive features. Our objective was to assess the feasibility and accuracy of a novel RNA-based blood assay to identify individuals with a high-risk tumor mutation in patients with PTC. Patients with benign or malignant thyroid disorders were included between September 2013 and July 2014 before either thyroidectomy (n£¿=£¿62) or treatment of recurrent or metastatic PTC (n£¿=£¿8). RNA was isolated from peripheral blood lymphocytes and reverse transcribed and followed by two rounds of nested PCR amplification with a restriction digest specific for wild-type BRAF. %U https://jmd.amjpathol.org/article/S1525-1578(15)00223-8/fulltext