%0 Journal Article
%T Quantification of the Mutant CALR Allelic Burden by Digital PCR: Application to Minimal Residual Disease Evaluation after Bone Marrow Transplantation
%A Arnaud Saint-L¨¦zer
%A Audrey Bidet
%A Bruno Cassinat
%A Chlo¨¦ James
%A Emmanuelle Verger
%A Eric Lippert
%A Fran£¿ois-Xavier Mahon
%A G¨¦rard Soci¨¦
%A Marie Robin
%A Marina Migeon
%A Olivier Mansier
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2015.07.007
%X With the recent discovery of CALR mutations, >80% of patients with myeloproliferative
neoplasms carry a phenotype-driving mutation. For JAK2 V617F, the most frequent mutation
in myeloproliferative neoplasms, accurate determination of mutational loads is of
interest at diagnosis, for phenotypic and prognostic purposes, and during follow-up
for minimal residual disease assessment. We developed a digital PCR technique that
allowed the accurate determination of CALR allelic burdens for the main mutations
(types 1 and 2).
%U https://jmd.amjpathol.org/article/S1525-1578(15)00219-6/fulltext