%0 Journal Article
%T Effects of Collection and Processing Procedures on Plasma Circulating Cell-Free DNA from Cancer Patients
%A Andrea Ruiz-Valdepenas Martin de Almagro
%A Anna Piskorz
%A Bente Risberg
%A Carlos Caldas
%A Charlotte Hodgkin
%A Christine Parkinson
%A Dana W.Y. Tsui
%A Davina Gale
%A Dineika Chandrananda
%A Elizabeth Moore
%A Francesco Marass
%A Heather Biggs
%A James D. Brenton
%A James Morris
%A Linda Jones
%A Nitzan Rosenfeld
%A Sarah-Jane Dawson
%A Vincent Plagnol
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.07.005
%X Circulating tumor DNA (ctDNA) offers new opportunities for noninvasive cancer management.
Detecting ctDNA in plasma is challenging because it constitutes only a minor fraction
of the total cell-free DNA (cfDNA). Pre-analytical factors affect cfDNA levels contributed
from leukocyte lysis, hence the ability to detect low-frequency mutant alleles. This
study investigates the effects of the delay in processing, storage temperatures, different
blood collection tubes, centrifugation protocols, and sample shipment on cfDNA levels.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30625-6/fulltext