%0 Journal Article
%T Detection of EGFR Variants in Plasma: A Multilaboratory Comparison of a Real-Time PCR EGFR Mutation Test in Europe
%A Alessandra Sacco
%A Antonio Marchetti
%A Boe Sorensen
%A Cleo Keppens
%A David Gonzalez de Castro
%A Ed Schuuring
%A Elisabeth M.C. Dequeker
%A Fernando Lopez-Rios
%A Francesca Fenizia
%A Hana Vosmikova
%A Izidor Kern
%A J. Han van Krieken
%A John F. Palma
%A Maria D. Lozano
%A Markus Tiemann
%A Nicola Normanno
%A Partha M. Das
%A Philippe Halfon
%A Phillipe Taniere
%A Selma H£¿nigschnabl
%A Sidney Scudder
%A Ulrike Setinek
%A Wei Wen
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.03.006
%X Molecular testing of EGFR is required to predict the response likelihood to targeted
therapy in non¨Csmall cell lung cancer. Analysis of circulating tumor DNA in plasma
may complement limitations of tumor tissue. This study evaluated the interlaboratory
performance and reproducibility of a real-time PCR EGFR mutation test (cobas EGFR
Mutation Test v2) to detect EGFR variants in plasma. Fourteen laboratories received
two identical panels of 27 single-blinded plasma samples. Samples were wild type or
spiked with plasmid DNA to contain seven common EGFR variants at six predefined concentrations
from 50 to 5000 copies per milliliter.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30514-7/fulltext