%0 Journal Article
%T Droplet Digital PCR Is a Robust Tool for Monitoring Minimal Residual Disease in Adult Philadelphia-Positive Acute Lymphoblastic Leukemia
%A Angela Minervini
%A Antonella Zagaria
%A Claudia Brunetti
%A Cosimo Cumbo
%A Crescenzio F. Minervini
%A Elisa Parciante
%A Francesco Albano
%A Giorgina Specchia
%A Giuseppina Tota
%A Luciana Impera
%A Luisa Anelli
%A Nicoletta Coccaro
%A Paola Carluccio
%A Paola Casieri
%A Paola Orsini
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.03.002
%X The breakpoint cluster region¨Cabelson 1 p190 fusion transcript is the most frequent
variant observed in Philadelphia-positive (Ph+) acute lymphoblastic leukemia (ALL).
Qualitative-PCR and real-time quantitative PCR are the currently used methods to monitor
minimal residual disease (MRD) in Ph+£żALL patients; for the latter, full standardization
and an international quality validation are lacking. Here, we developed a droplet
digital PCR (ddPCR) assay for MRD monitoring in p190+£żALL cases. The analytical performance
was assessed by the limit-of-detection determination, showing a reliability, sensitivity,
and precision of the assay of up to 0.001%.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30597-4/fulltext