%0 Journal Article
%T Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations
%A Diana Y. Vargas
%A Fred R. Kramer
%A Salvatore A.E. Marras
%A Sanjay Tyagi
%J The Journal of Molecular Diagnostics
%D 2018
%R 10.1016/j.jmoldx.2018.03.004
%X In PCR assays designed to detect rare somatic mutations, SuperSelective primers, by
virtue of their short 3¡ä-foot sequences, selectively initiate synthesis on mutant
DNA target fragments, while suppressing the synthesis of related wild-type fragments,
and the resulting threshold cycle reflects the quantity of mutant targets present.
However, when there are ¡Ü10 mutant target fragments in a sample, the threshold cycle
that is observed occurs so late that it can be confused with the threshold cycle that
arises from samples that contain only abundant related wild-type fragments.
%U https://jmd.amjpathol.org/article/S1525-1578(17)30442-7/fulltext