%0 Journal Article
%T Driver Gene Mutations in Stools of Colorectal Carcinoma Patients Detected by Targeted Next-Generation Sequencing
%A Arto Kokkola
%A Gemma Armengol
%A Masoud Doghaei-Moghaddam
%A Masoud Sotoudeh
%A Pauli Puolakkainen
%A Reza Ansari
%A Reza Ghanbari
%A Reza Malekzadeh
%A Sakari Knuutila
%A Virinder K. Sarhadi
%J The Journal of Molecular Diagnostics
%D 2016
%R 10.1016/j.jmoldx.2016.01.008
%X Detection of driver gene mutations in stool DNA represents a promising noninvasive
approach for screening colorectal cancer (CRC). Amplicon-based next-generation sequencing
(NGS) is a good option to study mutations in many cancer genes simultaneously and
from a low amount of DNA. Our aim was to assess the feasibility of identifying mutations
in 22 cancer driver genes with Ion Torrent technology in stool DNA from a series of
65 CRC patients. The assay was successful in 80% of stool DNA samples. NGS results
showed 83 mutations in cancer driver genes, 29 hotspot and 54 novel mutations.
%U https://jmd.amjpathol.org/article/S1525-1578(16)30028-9/fulltext