%0 Journal Article
%T Cytokine-Induced Killer Cells Modulates Resistance to Cisplatin in the A549/DDP Cell Line
%A Chunjuan Du
%A Hui Li
%A Jinpu Yu
%A Lei Wu
%A Lili Yang
%A Shui Cao
%A Wenwen Yu
%A Xiubao Ren
%A Xiumei An
%J Journal of Cancer
%D 2017
%I Ivyspring International Publisher
%R 10.7150/jca.19426
%X Background Cytokine-induced killer (CIK) cells can potentially enhance the tumor-killing activity of chemotherapy. Objective This study aimed to evaluate the effects of CIK cells on cisplatin (DDP) resistance in the human lung adenocarcinoma cell line A549/DDP. Methods The detect resistance index, drug resistance related-genes and cytokine secretion of A549/DDP co-cultured with CIK cells were assayed in vitro. Results After A549/DDP co-culture with CIK cells, the DDP resistance of A549/DDP significantly decreased in a time-dependent manner. The DDP resistance of A549/DDP co-cultured with CIK cells for 20 h decreased 4.93-fold compared with that of A549/DDP cells cultured alone (P<0.05). The mRNA and protein expression levels of the glutathione-S-transferase (GST) -¦Ð gene in A549/DDP significantly decreased after co-culture with CIK cells (P<0.05). The secretion of interferon (IFN)- ¦Ã significantly increased along with the co-culture time of A549/DDP with CIK cells. The expression of GST-¦Ð was restored by adding the neutralizing IFN-¦Ã. Conclusion CIK cells can reverse the drug resistance of A549/DDP in a time-dependent manner by reducing GST-¦Ð expression to increase the accumulation of DDP. The effect of CIK cells on re-sensitizing lung cancer cells to the chemotherapy drug was partially dependent on the secretion of IFN-¦Ã.
%K cytokine-induced killer cells
%K chemotherapy resistance
%K lung cancer
%K GST-¦Ð
%K IFN-¦Ã.
%U http://www.jcancer.org/v08p3287.htm