%0 Journal Article
%T Visualization of Macrophage Recruitment to Inflammation Lesions using Highly Sensitive and Stable Radionuclide-Embedded Gold Nanoparticles as a Nuclear Bio-Imaging Platform
%A Byeong-Cheol Ahn
%A Dong-Kwon Lim
%A Ho Won Lee
%A In-Kyu Lee
%A Jaetae Lee
%A Sang Bong Lee
%A Sang Kyoon Kim
%A Sang-Woo Lee
%A Sangil Choi
%A Shin Young Jeong
%A Sung Jin Cho
%A Thoudam Debraj Singh
%A Yinghua Li
%A Yong Hyun Jeon
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.17131
%X Reliable and sensitive imaging tools are required to track macrophage migration and provide a better understating of their biological roles in various diseases. Here, we demonstrate the possibility of radioactive iodide-embedded gold nanoparticles (RIe-AuNPs) as a cell tracker for nuclear medicine imaging. To demonstrate this utility, we monitored macrophage migration to carrageenan-induced sites of acute inflammation in living subjects and visualized the effects of anti-inflammatory agents on this process. Macrophage labeling with RIe-AuNPs did not alter their biological functions such as cell proliferation, phenotype marker expression, or phagocytic activity. In vivo imaging with positron-emission tomography revealed the migration of labeled macrophages to carrageenan-induced inflammation lesions 3 h after transfer, with highest recruitment at 6 h and a slight decline of radioactive signal at 24 h; these findings were highly consistent with the data of a bio-distribution study. Treatment with dexamethasone (an anti-inflammation drug) or GSK5182 (an ERR¦Ã inverse agonist) hindered macrophage recruitment to the inflamed sites. Our findings suggest that a cell tracking strategy utilizing RIe-AuNPs will likely be highly useful in research related to macrophage-related disease and cell-based therapies.
%K gold nanoparticles
%K nuclear bio-imaging platform
%K macrophage migration
%K acute inflammation.
%U http://www.thno.org/v07p0926.htm