%0 Journal Article
%T The Bidirectional Regulation between MYL5 and HIF-1¦Á Promotes Cervical Carcinoma Metastasis
%A Hai-Feng Gu
%A Jing Xu
%A Lan Zhang
%A Lin-Jing Yuan
%A Long Huang
%A Min Zheng
%A Rong-Zhen Luo
%A Shu-Ting Huang
%A Ting Wan
%A Wei-Hua Jia
%A Xing-Juan Yu
%A Yan-Ling Feng
%A Yun Zhou
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.20796
%X Myosin light chains (MLC) serve important regulatory functions in a wide range of cellular and physiological processes. Recent research found that MLC are also chromatin-associated nuclear proteins which regulate gene transcription. In this study, the MLC member myosin regulatory light chain 5 (MYL5) expression was upregulated in late stage cervical cancer patients, positively correlated with pelvic lymph node metastasis, and identified as a poor survival indicator. MYL5 overexpression promoted metastasis in cervical cancer in vitro and in vivo models, whereas MYL5 silencing had the converse effect. We demonstrated a bidirectional regulation between MYL5 and hypoxia inducible factor-1¦Á (HIF-1¦Á). HIF-1¦Á activates MYL5 via binding to the hypoxia response element (HRE) in the promoter of MYL5, and MYL5 could sustain HIF-1¦Á expression by tethering to recognition sequence AGCTCC in the HIF-1¦Á promoter region. Clinical data confirmed a positive correlation between MYL5 and HIF-1¦Á. In summary, our data show that MYL5 may act as a prognosis predictive factor in cervical carcinoma, and strategies that inhibit the interaction of MYL5 and HIF-1¦Á may benefit the cervical carcinoma patients with metastasis.
%K MYL5
%K cervical cancer
%K oxygen regulation.
%U http://www.thno.org/v07p3768.htm