%0 Journal Article
%T Evaluation of antibody fragment properties for near-infrared fluorescence imaging of HER3-positive cancer xenografts
%A Ayman El-Sayed
%A C. Ronald Geyer
%A Carolina Gonzalez
%A Humphrey Fonge
%A Kris Barreto
%A Landon Pastushok
%A Wayne Hill
%A Wendy Bernhard
%J Theranostics
%D 2018
%I Ivyspring International Publisher
%R 10.7150/thno.24252
%X In vivo imaging is influenced by the half-life, tissue penetration, biodistribution, and affinity of the imaging probe. Immunoglobulin G (IgG) is composed of discrete domains with known functions, providing a template for engineering antibody fragments with desired imaging properties. Here, we engineered antibody-based imaging probes, consisting of different combinations of antibody domains, labeled them with the near-infrared fluorescent dye IRDye800CW, and evaluated their in vivo imaging properties. Antibody-based imaging probes were based on an anti-HER3 antigen binding fragment (Fab) isolated using phage display.
%K antibody fragments
%K near-infrared fluorescence imaging
%K HER3
%K ErbB3
%K IRDye800CW
%U http://www.thno.org/v08p4856.htm