%0 Journal Article
%T MicroRNA-647 Targets SRF-MYH9 Axis to Suppress Invasion and Metastasis of Gastric Cancer
%A Baoxiong Zhuang
%A Da Wang
%A Gengtai Ye
%A Guoxin Li
%A Haijun Deng
%A Haipeng Zhou
%A Hao Liu
%A Jiang Yu
%A Kunzhai Huang
%A Lei Zhang
%A Li Yan
%A Liying Zhao
%A Qingbin Yang
%A Wende Li
%A Xianjun Zhu
%A Xiaolong Qi
%A Yanfeng Hu
%A Yuming Jiang
%A Zhiyong Shen
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.20512
%X MicroRNAs (miRNAs) play important roles in regulating tumour development and progression. Here we show that miR-647 is repressed in gastric cancer (GC), and associated with GC metastasis. Moreover, we identify that miR-647 can suppress GC cell migration and invasion in vitro. Mechanistically, we confirm miR-647 directly binds to the 3' untranslated regions of SRF mRNA, and SRF binds to the CArG box located at the MYH9 promoter. CCG-1423, an inhibitor of RhoA/SRF-mediated gene transcription, inhibits the expression of MYH9, especially in SRF downregulated cells. Overexpression of miR-647 inhibits MGC 80-3 cells' metastasis in orthotropic GC models, but increasing SRF expression in these cells reverses this change. Importantly, we found the synergistic inhibition effect of CCG-1423 and agomir-647, an engineered miRNA mimic, on cancer metastasis in orthotropic GC models. Our study demonstrates that miR-647 functions as a tumor metastasis suppressor in GC by targeting SRF/MYH9 axis.
%K Gastric cancer
%K Metastasis
%K MicroRNA-647
%K MYH9
%K SRF.
%U http://www.thno.org/v07p3338.htm