%0 Journal Article
%T Fluorocarbons Enhance Intracellular Delivery of Short STAT3-sensors and Enable Specific Imaging
%A Alexei Bogdanov Jr.
%A Anand T.N. Kumar
%A Shaokuan Zheng
%A Surong Zhang
%A Valeriy Metelev
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.19704
%X Short oligonucleotide sequences are now being widely investigated for their potential therapeutic properties. The modification of oligonucleotide termini with short fluorinated residues is capable of drastically altering their behavior in complex in vitro and in vivo systems, and thus may serve to greatly enhance their therapeutic potential. The main goals of our work were to explore: 1) how modification of STAT3 transcription factor-binding oligodeoxynucleotide (ODN) duplexes (ODND) with one or two short fluorocarbon (FC)-based residues would change their properties in vitro and in vivo, and if so, how this would affect their intracellular uptake by cancer cells, and 2) the ability of such modified ODND to form non-covalent complexes with FC-modified carrier macromolecule. The latter has an inherent advantage of producing a 19F-specific magnetic resonance (MR) imaging signature. Thus, we also tested the ability of such copolymers to generate 19F-MR signals.
%K fluorocarbon
%K oligonucleotide
%K magnetic resonance imaging
%K confocal microscopy
%K STAT3.
%U http://www.thno.org/v07p3354.htm