%0 Journal Article
%T 3,3¡ä-Diindolylmethane stimulates exosomal Wnt11 autocrine signaling in human umbilical cord mesenchymal stem cells to enhance wound healing
%A Aihua Gong
%A Bin Zhang
%A Fei Mao
%A Hui Qian
%A Hui Shi
%A Jiahao Xu
%A Rong Li
%A Wenrong Xu
%A Xiao Xu
%A Xu Zhang
%A Yaoxiang Sun
%A Yongmin Yan
%A Zhaoji Pan
%J Theranostics
%D 2017
%I Ivyspring International Publisher
%R 10.7150/thno.18082
%X Human umbilical cord-derived mesenchymal stem cells (hucMSCs) are suggested as a promising therapeutic tool in regenerative medicine, however, their efficacy requires improvement. Small molecules and drugs come up to be a convenient strategy in regulating stem cells fate and function. Here, we evaluated 3,3¡ä-diindolylmethane (DIM), a natural small-molecule compound involved in the repairing effects of hucMSCs on a deep second-degree burn injury rat model. HucMSCs primed with 50 ¦ÌM of DIM exhibited desirable repairing effects compared with untreated hucMSCs. DIM enhanced the stemness of hucMSCs, which was related to the activation of Wnt/¦Â-catenin signaling. ¦Â-catenin inhibition impaired the healing effects of DIM-primed hucMSCs (DIM-hucMSCs) in vivo. Moreover, we demonstrated that DIM upregulated Wnt11 expression in hucMSC-derived exosomes. Wnt11 knockdown inhibited ¦Â-catenin activation and stemness induction in DIM-hucMSCs and abrogated their therapeutic effects in vivo. Thus, our findings indicate that DIM promotes the stemness of hucMSCs through increased exosomal Wnt11 autocrine signaling, which provides a novel strategy for improving the therapeutic effects of hucMSCs on wound healing.
%K 3
%K 3¡ä-diindolylmethane
%K wound healing
%K Wnt11
%K exosome.
%U http://www.thno.org/v07p1674.htm