%0 Journal Article
%T 沉默STAT3增强SNX-2112诱导食管癌干细胞凋亡的研究
%A 徐单单
%A 王颖
%A 陈素红
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1004
%X 摘要 目的 通过沉默STAT3探讨Hsp90抑制剂SNX-2112对食管癌干细胞的凋亡诱导效果。方法 构建干扰STAT3的慢病毒载体shSTAT3并转染食管癌干细胞，同时设计阴性对照组，SNX-2112作用于细胞，CCK8法分析shSTAT3与SNX-2112联用对食管癌干细胞的活性抑制效果；软琼脂平板克隆实验研究二者联用对细胞的增殖抑制效果；流式细胞仪检测并分析二者联用对食管癌干细胞的凋亡诱导效果；Western blot分析凋亡蛋白变化。结果 设计并构建的针对STAT3的慢病毒载体shSTAT3能在蛋白（P<0.01）及mRNA水平（P<0.05）有效干扰其表达；经过shSTAT3及0.015625、0.03125、0.0625、0.125、0.25、0.5和1.0 μmol/L的SNX-2112作用24 h后，细胞活性较单用组明显下降（P<0.05），同时SNX-2112的IC50为（0.28±0.01）μmol/L；克隆形成实验显示，二者联用导致细胞克隆形成效率明显下降（P<0.01）；流式细胞仪结果显示，二者联用后细胞凋亡比例以及subG1比例细胞数量显著提高（P<0.01, P<0.01）；Western blot结果显示，shSTAT3及SNX-2112联用后抗凋亡蛋白Bcl2表达水平下调（P<0.05），促凋亡蛋白Bax水平上调（P<0.05）。结论 沉默STAT3增强了Hsp90抑制剂SNX-2112诱导食管癌干细胞凋亡的效果
%K STAT3
%K 热休克蛋白90
%K SNX-2112
%K 食管癌干细胞
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%U http://www.zlfzyj.com/CN/abstract/abstract9348.shtml