%0 Journal Article
%T 抑制LITAF基因对人脑胶质母细胞瘤U251细胞增殖、凋亡和放疗敏感度的影响
%A 朱虹帆
%A 李光辉
%A 汤金梁
%A 王东林
%A 陈典刚
%A 黄昌林
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1031
%X 摘要 目的 分析人脑胶质母细胞瘤组织中LITAF基因mRNA表达情况，并通过抑制人脑胶质母细胞瘤U251细胞中LITAF基因表达，观察其对U251细胞增殖、凋亡和放疗敏感度的影响。方法 分析TCGA数据库中人脑胶质母细胞瘤组织中LITAF基因mRNA表达；通过RNAi抑制U251细胞中LITAF基因表达，采用胸腺嘧啶核苷类似物（EDU）检测U251细胞增殖变化，流式细胞仪分析U251细胞凋亡的变化，克隆形成实验和流式细胞分析U251细胞放疗敏感度的变化。 结果 TCGA数据库分析结果显示人脑胶质母细胞瘤组织LITAF表达显著高于正常脑组织；抑制LITAF表达后，U251细胞的增殖和凋亡未见明显变化，但在电离辐射后，LITAF抑制组U251细胞凋亡显著低于对照组，克隆形成显著高于对照组，对放疗敏感度减弱。结论 LITAF基因高表达于人脑胶质母细胞瘤组织，抑制人脑胶质母细胞瘤U251细胞中LITAF基因表达不影响细胞的增殖和凋亡，但显著降低细胞的放疗敏感度
%K 脑胶质母细胞瘤
%K LITAF
%K U251细胞
%K 增殖
%K 凋亡
%K 放疗敏感度
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway
%K Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3
%K Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line
%K Effects of Pachymaran on Proliferation
%K Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism
%K Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells
%K Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition
%K Effects of NDUFA4 Overexpression on Growth of Human Colon Cancer Cells in vitro
%U http://www.zlfzyj.com/CN/abstract/abstract9329.shtml