%0 Journal Article
%T TAK-242对人多发性骨髓瘤细胞增殖及凋亡的影响
%A 段萍萍
%A 艾丽梅
%J 肿瘤防治研究
%D 2019
%R 10.3971/j.issn.1000-8578.2019.18.1743
%X 摘要 目的 探讨Toll样受体4（TLR4）特异性抑制剂TAK-242对人多发性骨髓瘤细胞株RPMI8226增殖及凋亡的影响。方法 取对数期生长的人多发性骨髓瘤细胞株RPMI8226分为A、B、C组及对照组（Control），A、B、C组分别加入终浓度为20、40、80 μmol/L的TAK-242，对照组不加抑制剂，培养24 h后，采用CCK-8法检测细胞增殖抑制率；Annexin-V/PI检测细胞凋亡率；RT-PCR检测细胞TLR4、Myd88 mRNA的表达水平；Western blot检测细胞Myd88、NF-κB的蛋白表达情况。结果 A、B、C组细胞增殖抑制率、凋亡率升高，TLR4、Myd88 mRNA表达水平及Myd88、NF-κB的蛋白表达水平降低，各浓度组之间的差异均有统计学意义（P<0.05）。结论 TAK-242可抑制人多发性骨髓瘤细胞RPMI8226的增殖，促进细胞凋亡，其机制可能为NF-κB信号通路功能被抑制
%K 多发性骨髓瘤
%K TAK-242
%K 增殖
%K 凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9373.shtml