%0 Journal Article
%T 长链非编码RNA-p21诱导脑动脉粥样硬化血管平滑肌细胞凋亡<br>Long-chain non-coding RNA-p21 induces apoptosis of vascular smooth muscle cells in cerebral atherosclerosis
%A 李 昌
%A 唐翠娥
%A 付 蓉
%A 任思颖
%A 伍国锋
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201902009
%X 摘要：目的 探究长链非编码RNA-p21(lncRNA-p21)通过调控脑平滑肌细胞凋亡，参与脑动脉粥样硬化发生发展的机制。方法 选取贵阳市第二人民医院健康体检者和脑血管动脉粥样硬化患者各30例，实时荧光定量PCR(Real time PCR，RT-PCR)检测患者血清lncRNA-p21的含量。建立大鼠脑动脉粥样硬化模型，HE染色观察大鼠脑组织病理改变，RT-PCR检测脑组织lncRNA-p21含量，Western blot检测抗凋亡蛋白Bcl-2和促凋亡蛋白Bax以及mTOR的表达。用ox-LDL刺激人脑血管平滑肌细胞(BVSMCs)构建血管平滑肌细胞动脉粥样硬化模型，过表达或抑制lncRNA-p21，流式细胞仪和TUNEL检测细胞凋亡情况。Caspase-3活性检测试剂盒检测凋亡蛋白Caspase-3的表达活性。结果 脑动脉粥样硬化患者血清和模型组大鼠脑组织lncRNA-p21表达显著下降(P<0.05)；HE染色结果显示模型组大鼠脑组织损伤明显，lncRNA-p21减少(P<0.05)；同时伴有Caspase-3活性升高，Bcl-2、mTOR蛋白表达下降且Bax蛋白表达升高(P<0.05)；在细胞试验中，流式细胞仪和TUNEL结果显示过表达lncRNA-p21可以降低BVSMCs的凋亡，抑制lncRNA-p21促进BVSMCs的凋亡(P<0.05)；脑血管平滑肌细胞中过表达lncRNA-p21，Caspase-3活性降低，Bcl-2、mTOR蛋白表达升高且Bax蛋白表达降低(P<0.05)；抑制表达lncRNA-p21，Caspase-3活性增加，Bcl-2、mTOR蛋白表达降低且Bax蛋白表达升高(P<0.05)。结论 lncRNA-p21可能通过调控mTOR蛋白表达来调控脑血管平滑肌细胞凋亡，参与脑动脉粥样硬化的病理过程。<br>ABSTRACT: Objective To explore the mechanism of lncRNA-p21 regulating the apoptosis of cerebral smooth muscle cells (SMCs) involved in the occurrence and development of cerebral atherosclerosis. Methods We selected 30 subjects receiving physical checkup and 30 patients with cerebrovascular atherosclerosis in our hospital. The content of lncRNA-p21 in serum was detected by Real-time fluorescence quantitative PCR. The rat model of cerebral atherosclerosis was established. The pathological changes of rat brain were observed by HE staining. The content of lncRNA-p21 in brain tissue was detected by RT-PCR; Western blot was used to detect the expressions of mTOR, anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax. Vascular smooth muscle cells (BVSMCs) were stimulated with ox-LDL to establish atherosclerosis model of vascular SMCs. lncRNA-p21 was overexpressed or inhibited. Apoptosis was detected by flow cytometry and TUNEL. The Caspase-3 activity assay kit was used to detect the expression of Caspase-3. Western Blot was used to detect the expressions of mTOR, anti-apoptotic protein Bcl-2 and pro-apoptotic protein Bax. Results The expression of lncRNA-p21 was significantly decreased in patients with cerebral atherosclerosis and model group rats (P<0.05). HE staining showed that the decrease of lncRNA-p21 could contribute to the pathological changes of rat brain tissue accompanied by increased activity of Caspase-3, decreased expressions of Bcl-2 and mTOR protein and increased expression of Bax protein (P<0.05). Flow cytometry and TUNEL showed that overexpression of lncRNA-p21 could reduce the apoptosis of BVSMCs, inhibiting lncRNA-p21 could promote the apoptosis of BVSMCs (P<0.05). In VSMCs, lncRNA-p21 was overexpressed, Caspase-3 activity decreased, Bcl-2, mTOR protein expression
%K lncRNA-p21
%K 脑动脉粥样硬化
%K mTOR
%K 凋亡<br>lncRNA-p21
%K cerebral atherosclerosis
%K mTOR
%K apoptosis
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201902009