%0 Journal Article
%T 进展期胃癌脾脏巨噬细胞表型变化及其共培养对胃癌细胞增殖侵袭迁移的影响<br>Phenotypic changes of spleen macrophages in advanced gastric cancer and their effects on proliferation, invasion and migration of gastric cancer cells
%A 潘 敦
%A 李良庆
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201904008
%X 摘要：目的 探讨进展期胃癌脾脏巨噬细胞表型变化及体外诱导培养的M1型和M2型巨噬细胞与胃癌细胞共培养对癌细胞增殖侵袭迁移的影响，以期了解脾脏在胃癌发展中的作用。方法 收集福建医科大学附属第一医院2015年3月至2017年5月期间15例行进展期近端胃癌根治术联合脾脏切除术患者的新鲜手术切除脾脏组织，分离巨噬细胞，流式细胞仪检测各型巨噬细胞表型变化；全自动磁珠提取系统从健康志愿者新鲜血液中分离单核细胞，分别给予10ng/mL的GM-CSF和M-CSF诱导成M1型、M2型巨噬细胞，并用CD16和CD163进行鉴定；利用Transwell共培养法将胃癌细胞SGC-7901和AGS与M1、M2型巨噬细胞和单核细胞共培养，锥虫蓝(台盼蓝)染色进行计数来检测细胞增殖、侵袭迁移能力变化。结果 进展期胃癌患者脾脏中M2型巨噬细胞比例增加，M1型巨噬细胞比例降低；单核细胞经GM-CSF和M-CSF体外培养，可分别诱导为M1型、M2型巨噬细胞，其阳性率分别为(95.46±4.21)%和(94.67±4.97)%；M2型巨噬细胞和单核细胞可促进胃癌细胞增殖侵袭迁移，M1型巨噬细胞则抑制胃癌细胞增殖侵袭迁移。结论 本研究鉴定出进展期胃癌患者脾脏组织中不同巨噬细胞的比例，证实了不同类型巨噬细胞对胃癌细胞的影响不同，M2型巨噬细胞起促进胃癌细胞进展，而M1型巨噬细胞则对胃癌细胞起抑制作用，进一步揭示了脾脏在胃癌进展中的作用，为临床制订进展期胃癌的治疗方案提供了研究基础。<br>ABSTRACT: Objective To investigate the phenotypic changes of splenic macrophages in advanced gastric cancer and the effects of co-culture with M1 and M2 macrophages on gastric cancer cells in order to explore the role of the spleen in the development of gastric cancer. Methods We collected the fresh surgically removed spleens from 15 patients who had undergone advanced proximal gastrectomy combined with splenectomy in our hospital from March 2015 to May 2017. Spleen macrophages were isolated from these spleen tissues and the macrophage phenotypes were detected by flow cytometry. Monocytes were isolated from fresh blood of healthy volunteers using a fully automated bead extraction system; the identified monocytes were given 10ng/mL of GM-CSF and M-CSF to be induced into M1-type macrophages and M2-type macrophages, respectively. The phenotypes of macrophages were identified by flow cytometry using CD16 and CD163; the gastric cancer cells SGC-7901 and AGS were co-cultured with M1, M2 macrophages and monocytes using Transwell co-culture method; trypan blue staining was used to detect the proliferative, invasive and migratory abilities of the cells. Results The proportion of type M2 macrophages in the spleen of patients with advanced gastric cancer was increased while the proportion of type M1 macrophages decreased. The mononuclear cells were induced into M1 and M2 macrophages by culture with GM-CSF and M-CSF, and the positive rate of monocytes was (95.46±4.21)% and (94.67±4.97)%, respectively; M2 macrophages and monocytes could promote the invasion and migration of gastric cancer cells, while M1 macrophages inhibited the invasion and migration of gastric cancer cells. Conclusion This study identified the proportions of different macrophages in spleen tissues of patients with advanced gastric cancer, and confirmed that the different types of macrophages on gastric cancer cells have different effects. M2 macrophages promoted gastric
%K 胃癌
%K M1型巨噬细胞
%K M2型巨噬细胞
%K 增殖
%K 侵袭
%K 迁移<br>gastric cancer
%K M1 macrophage
%K M2 macrophage
%K proliferation
%K invasion
%K migration
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201904008