%0 Journal Article
%T 细胞周期蛋白依赖性激酶16(CDK16)与细胞增殖指标Ki67在食管鳞癌中的表达及意义<br>Expression and significance of cyclin-dependent kinase 16 and cell proliferation index Ki67 in esophageal squamous cell carcinoma
%A 郑 皓
%A 景嘉楠
%A 董 慧
%A 轩 阁
%A 李铁鹏
%A 夏 鑫
%A 董子明
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201903016
%X 摘要：目的 探讨细胞周期蛋白依赖性激酶16(CDK16)及细胞增殖指标Ki67在人食管鳞癌组织中的表达及其生物学意义，并探讨二者在食管鳞癌中表达的相关性。方法 采用免疫组织化学方法检测CDK16、Ki67蛋白在75例食管鳞癌/癌旁组织中的表达，使用数学统计分析软件分析这两种蛋白的表达与食管鳞癌临床病理特征之间的关系。结果 CDK16在食管鳞癌组织中的表达显著高于癌旁组织中的表达(P<0.05)，CDK16的表达与食管鳞癌的分化程度、淋巴结转移及TNM临床分期密切相关(P<0.05)，而与食管鳞癌患者的性别、年龄、肿瘤部位、肿瘤最大径及肿瘤的病理形态均无关(P>0.05)。另外，在食管鳞癌组织中，Ki67的表达与食管鳞癌的分化程度、淋巴结转移及TNM临床分期之间存在显著的相关性(P<0.05)，且相关性分析显示，食管鳞癌中CDK16与Ki67二者的表达呈显著正相关(χ2=24.36, P<0.001, Pearson列联系数=0.518)。结论 CDK16蛋白和Ki67蛋白在食管鳞癌中的表达明显升高，联合检测CDK16与Ki67的表达，可作为判断食管鳞癌恶性程度及评估患者预后水平的重要参考指标。<br>ABSTRACT: Objective To investigate the expression and biological significance of cyclin-dependent kinase 16 (CDK16) and cell proliferation index Ki67 in esophageal squamous cell carcinoma so as to explore the correlation between the expressions of these two proteins in esophageal squamous cell carcinoma. Methods Immunohistochemistry was used to detect the expressions of CDK16 protein and Ki67 protein in 75 cases of esophageal squamous cell carcinoma and paracancerous tissues. The relationship between the expressions of these two proteins and the clinicopathological features of esophageal squamous cell carcinoma was studied by using mathematical statistical analysis software. Results The expression of CDK16 in esophageal squamous cell carcinoma tissues was significantly higher than that in paracancerous tissues (P<0.05). The expression of CDK16 was closely related to the differentiation, lymph node metastasis and clinical stage of esophageal squamous cell carcinoma (P<0.05). However, it had no correlation with gender, age, tumor location, the largest diameter of tumor, or pathological morphology of esophageal squamous cell carcinoma (P>0.05). In addition, there was a significant correlation of Ki67 expression with differentiation, lymph node metastasis and TNM clinical stage of esophageal squamous cell carcinoma (P<0.05), and correlation analysis showed a significant positive correlation between CDK16 and Ki67 expressions in esophageal squamous cell carcinoma (χ2=24.36, P<0.001, Pearson coefficient of contingency =0.518). Conclusion The expressions of CDK16 protein and Ki67 protein in esophageal squamous cell carcinoma increased significantly. The combined detection of CDK16 and Ki67 expressions can be used as an important reference index for judging the malignancy of esophageal squamous cell carcinoma and evaluating patients？？ prognosis
%K 食管鳞癌
%K 细胞周期蛋白依赖性激酶16(CDK16)
%K Ki67
%K 组织芯片
%K 免疫组化<br>esophageal squamous cell carcinoma
%K cyclin-dependent kinase 16
%K Ki67
%K tissue microarray
%K immunohistochemistry
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201903016