%0 Journal Article
%T siRNA下调AEG-1基因表达对胶质瘤细胞血管拟态形成的影响<br>Effects of down-regulation of astrocyte elevated gene-1 expression by siRNA on glioma vasculogenic mimicy
%A 梁 晨
%A 唐荣华
%A 上官健
%A 廉民学
%A 郭世文
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201901016
%X 摘要：目的 探讨下调星型细胞上调基因-1(AEG-1)表达对胶质瘤细胞血管拟态(VM)形成的影响及其可能的机制。方法 利用siRNA下调U87胶质瘤细胞中AEG-1表达，通过体外构建胶质瘤VM模型，研究下调AEG-1表达对胶质瘤VM形成的影响；Real-time PCR及Western blot分别检测AEG-1表达下调对U87细胞中血管内皮生长因子(VEGF)、基质金属蛋白酶-2(MMP2) mRNA及蛋白表达的影响；免疫荧光法检测AEG-1表达下调对VM模型中VEGF及MMP2表达的影响。结果 siRNA下调AEG-1表达能显著降低U87细胞体外VM的形成(P<0.01)，同时下调AEG-1基因能够显著抑制U87细胞及其构成的VM中VEGF及MMP2的表达(P<0.01)。结论 siRNA下调AEG-1表达能显著抑制胶质瘤细胞VM的形成，其部分机制可能是通过下调VEGF及MMP2表达实现。<br>ABSTRACT: Objective To investigate the effects of down-regulation of astrocyte elevated gene-1(AEG-1) expression by siRNA on glioma vasculogenic mimicy in vitro. Methods The expression of AEG-1 in U87 glioma cells was down-regulated by siRNA. An in vitro model of glioma vasculogenic mimicy was established to evaluate the effect of down-regulation of AEG-1 expression on glioma vasculogenic mimicy. Real-time PCR and Western blot were used to detect the mRNA and protein expressions of VEGF and MMP2 in U87 cells after treated with AEG-1 siRNA, respectively. Immunofluorescence was used to detect the expressions of VEGF and MMP2 in glioma vasculogenic mimicy model in vitro. Results Down-regulation of AEG-1 expression by siRNA could significantly inhibit the glioma vasculogenic mimicy in vitro(P<0.01). Meanwhile, It could also inhibit the expressions of VEGF and MMP2 in glioma cells and glioma vasculogenic mimicy in vitro (P<0.01). Conclusion These results suggest that down-regulation of AEG-1 expression by siRNA can significantly inhibit the glioma vasculogenic mimicy in vitro, and these effects may be realized partly through regulation of VEGF and MMP2 expression
%K 星型细胞上调基因-1
%K siRNA
%K 胶质瘤
%K 血管拟态<br>AEG-1
%K siRNA
%K glioma
%K vasculogenic mimicy
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201901016