%0 Journal Article
%T CTC检测在胃癌结直肠癌预后评估中的价值及差异性分析<br>Difference of CTC detection in the prognosis of gastric cancer and colorectal cancer
%A 赵 琳
%A 刘医辉
%A 董春慧
%A 张文文
%A 段学琴
%A 刘锡枝
%A 陈 玲
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201906019
%X 摘要：目的 探讨外周血循环肿瘤细胞(CTCs)检测对胃癌结直肠癌的预后价值及差异。方法 采用阴性富集联合免疫荧光原位杂交(imFISH)检测平台检测32例胃癌和36例结直肠癌患者CTCs细胞数，并进行两组间比较。结果 胃癌组患者外周血中检出CTCs<2组有8例(25%)，随访结束时，全部患者存活；检出CTCs≥2组有24例(75%)，仅有8例存活，两组的生存差异具有统计学意义(P=0.001)。在结直肠癌组患者中外周血检出CTCs<2组有28例(78%)，随访时间结束时，全部患者存活；检出CTCs≥2组有8例(22%)，其中6例患者在随访终点时间存活，结直肠癌中CTCs<2组与CTCs≥2组的生存差异具有统计学意义(P=0.044)。胃癌与结直肠癌的生存差异具有统计学意义(P=0.000)。CTCs检出结果与胃癌结直肠癌患者的性别、年龄、淋巴结转移、远处器官转移、病理分级均无关(P>0.05)。结论 采用阴性富集和imFISH技术检测胃癌和结直肠癌患者外周血CTCs，CTCs值升高的患者预后不良，且胃癌较结直肠癌预后不良。<br>ABSTRACT: Objective To investigate the clinical significance and difference of peripheral blood circulating tumor cells (CTCs) detection in the prognosis of patients with gastric cancer and colorectal cancer. Methods The CTCs in the peripheral blood of 32 patients with gastric cancer and 36 patients with colorectal cancer were detected by negative enrichment and imFISH technique, and the results were compared between the two groups. Results There were 8 cases (25%) of CTCs < 2 in the peripheral blood of gastric cancer patients. At the end of the follow-up, all patients survived; 24 cases (75%) of CTCs >2 were detected, and only 8 cases survived. The two groups differed significantly in survival (P=0.001). 28 cases (78%) of the patients with colorectal cancer were detected CTCs < 2 groups in the peripheral blood. At the end of the follow-up, all patients survived. 8 cases (22%) of the patients with CTCs > 2 groups survived at the end of the follow-up. 6 patients survived at the end of the follow-up. There was a significant difference between CTCs < 2 groups and CTCs > 2 groups in colorectal cancer (P=0.044). The survival in gastric cancer and colorectal cancer was significantly different (P=0.000). The results of CTCs were not correlated with gender, age, lymph node metastasis, distant organ metastasis or pathological grade of patients with colorectal cancer and gastric cancer (all P>0.05). Conclusion Negative enrichment and imFISH technique can be used to detect CTCs of the peripheral blood of patients with gastric cancer and colorectal cancer. The prognosis of patients with elevated CTCs is poor. Gastric cancer has a poorer prognosis than colorectal cancer
%K 胃癌
%K 结直肠癌
%K 循环肿瘤细胞
%K 阴性富集技术
%K imFISH技术<br>gastric cancer
%K colorectal cancer
%K circulating tumor cell
%K negative enrichment technique
%K imFISH technique
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201906019