%0 Journal Article
%T 白藜芦醇通过诱导细胞周期阻滞及细胞凋亡增强宫颈癌细胞的化疗敏感性<br>Resveratrol elevates the chemosensitivity of cervical cancer cells by inducing cell cycle arrest and apoptosis
%A 岳小丁
%A 李秀娟
%A 刘 青
%A 杨捷玲
%J 西安交通大学学报(医学版)
%D 2019
%R 10.7652/jdyxb201905014
%X 摘要：目的 探索白藜芦醇(resveratrol)对宫颈癌细胞化疗敏感性的影响及其调控机制。方法 Hela/DDP细胞分为4组：Hela/DDP组，resveratrol组，顺铂(cisplatin, CDDP)组，resveratrol+CDDP组。CCK-8检测细胞活力及增殖。流式细胞仪检测细胞周期及细胞凋亡。Western blot检测p21、CDK2、cyclinD1、caspase-9、caspase-3、Bcl-2和Bax表达。结果 Hela/DDP细胞对CDDP的敏感性低于Hela细胞(P<0.01)。白藜芦醇可增强Hela/DDP细胞对CDDP的敏感性(P<0.01)。白藜芦醇可提高CDDP对Hela/DDP细胞增殖的抑制作用(P<0.01)。白藜芦醇可增强CDDP对Hela/DDP细胞G1期阻滞作用(P<0.01)。resveratrol组和CDDP组p21表达高于Hela/DDP组，CDK2和cyclinD1低于Hela/DDP组(P<0.01)。与CDDP组相比，resveratrol+CDDP组p21表达升高，CDK2和cyclinD1表达降低(P<0.01)。白藜芦醇可提高CDDP对Hela/DDP 细胞凋亡的诱导作用(P<0.01)。resveratrol组和CDDP组caspase-3、caspase-9和Bax表达高于Hela/DDP组，Bcl-2表达低于Hela/DDP组(P<0.01)。与CDDP组相比，resveratrol+CDDP组caspase-3、caspase-9和Bax表达升高，Bcl-2表达下降(P<0.01)。结论 白藜芦醇可能通过诱导细胞周期阻滞及细胞凋亡而增强宫颈癌细胞的化疗敏感性。<br>ABSTRACT: Objective To explore the effect of resveratrol on the chemosensitivity of cervical cancer cells and its regulatory mechanism. Methods Hela/DDP cells were divided into four groups: Hela/DDP group, resveratrol group, CDDP group, and resveratrol+CDDP group. Cell viability and proliferation were detected by CCK-8. Cell cycle and apoptosis were tested by flow cytometry. The expressions of p21, CDK2, cyclinD1, caspase-9, caspase-3, Bcl 2 and Bax were measured by Western blot. Results The sensitivity of Hela/DDP cells to cisplatin was lower than that of Hela cells (P<0.01). Resveratrol significantly enhanced Hela/DDP cells’ sensitivity to cisplatin (P<0.01). The inhibition of CDDP on the proliferation of Hela/DDP cells was increased by resveratrol (P<0.01). The G1 phase arrest of CDDP on Hela/DDP cells was enhanced by resveratrol (P<0.01). The expression of p21 was higher in resveratrol group and CDDP group than in Hela/DDP group, and the expressions of CDK2 and CyclinD1 were lower than in Hela/DDP group (P<0.01). Compared with CDDP group, the expression of p21 in resveratrol+CDDP group was elevated with the decrease in CDK2 and cyclinD1 expressions (P<0.01). The induction of CDDP on the apoptosis of Hela/DDP cells was elevated by resveratrol (P<0.01). The expressions of caspase-3, caspase-9 and Bax were higher in resveratrol group and CDDP group than in Hela/DDP group, and the expression of Bcl-2 was lower than in Hela/DDP group (P<0.01). Compared with CDDP group, apoptosis in resveratrol+CDDP group was increased with the reduced expression of Bcl-2 (P<0.01). Conclusion Resveratrol enhances the chemosensitivity of cervical cancer cells by inducing cell cycle arrest and apoptosis
%K 宫颈癌
%K 白藜芦醇
%K 化疗敏感性
%K 细胞周期阻滞
%K 细胞凋亡<br>cervical cancer
%K resveratrol
%K chemosensitivity
%K cell cycle arrest
%K apoptosis
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201905014